Keratinocyte Growth Factor 2, Recombinant, Human (KGF2)

KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. \n\nRecombinant Human KGF-2 (FGF-10) is a single, non-glycosylated polypeptide chain containing 170 amino acids and having a molecular mass of 19300 Dalton. rHuKGF-2 is highly related to KGF-1(FGF-7), it binds to the same receptor as KGF-1 and shares 57% sequence homology. \n\nSequence :\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Leu-Glu-Gln-Asp. \n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis. \n\nBiological Activity:\nRecombinant Keratinocye Growth Factor-2 is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent stimulation of FGF recpetors by BaF3 indicator cells (measured by 3H-thymidine uptake) is less then 0.5ng/ml corresponding to a specific activity of 2 x 10e6 Units/mg. \n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of KGF-2. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 1.79 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of KGF-2 as a Reference Standard.\n\nReconstitution:\nReconstitute the lyophilized KGF-2 in sterile 18MOmega-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0201-15

Size

5ug

Form

Supplied as a lyophilized powder in 5mM sodium phosphate, pH 7.4, 80mM sodium chloride.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. FGF-10 induces SP-C and Bmp4 and regulates proximal-distal patterning in embryonic tracheal epithelium. Hyatt BA, Shangguan X, Am J Physiol Lung Cell Mol Physiol 2004 Dec;287(6):L1116-26 2. Roles of FGF-10 on the development of diathrodial limb joints. Lovinescu I, Koyama E, Penn Dent J (Phila) 2003;103:5, 9 3. Roles of fibroblast growth factor 10 (Fgf10) in adipogenesis in vivo. Asaki T, Konishi M, Kato S, Mol Cell Endocrinol 2004 Apr 15;218(1-2):119-28 4. Anatomical studies of the fibroblast growth factor-10 mutant, Sonic Hedge Hog mutant and androgen receptor mutant mouse genital tubercle. Yucel S, Liu W, Donjacour A, Adv Exp Med Biol 2004;545:123-48 5. Fibroblast growth factor 10 (Fgf10) invalidation results in anorectal malformation in mice. Fairbanks TJ, De Langhe S, Warburton D, J Pediatr Surg 2004 Mar;39(3):360-5; discussion 360-5 6. Fibroblast growth factor-10 attenuates H2O2-induced alveolar epithelial cell DNA damage: role of MAPK activation and DNA repair. Upadhyay D, Bundesmann M, Correa-Meyer E, Am J Respir Cell Mol Biol 2004 Jul;31(1):107-13.