Keratinocyte Growth Factor, Recombinant, Human (KGF, Fibroblast Growth Factor 7, FGF-7)

KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF. \n\nRecombinant Human KGF-1 (FGF-7) is a single, non-glycosylated, polypeptide chain containing 164 amino acids and having a molecular mass of 18995 Dalton. \n\nSequence:\nThe sequence of the first five N-terminal amino acids was determined and was found to be Met-Cys-Asn-Asp-Met. \n\nBiological Activity:\nKGF is fully biologically active when compared to standards. The ED50, calculated by the dose-dependent stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is < 10ng/ml corresponding to a specific activity of 10e5 Units/mg. Recombinant human KGF biological activity was measured by the KGF's mitogenic activity on BaF3 cells expressing the KGF receptor.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of KGF. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods: \n1. UV spectroscopy at 280nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard.\n\nReconstitution:\nReconstitute in sterile ddH2O to a concentration (same/more than) 100ug/ml, which can then be further diluted to other aqueous solutions. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Note: For long term storage it is recommended to add a carrier protein (0.1% BSA or HSA).

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SPECIFICATIONS

Catalog Number

K0201-01

Size

2ug

Form

Supplied as a lyophilized powder. No additives. Reconstitute in sterile ddH2O to a concentration (same/more than) 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified. Endotoxin: < 0.1ng/ug (IEU/ug).

References

1. Washizawa, N., et al., JPEN J. Parenter. Enteral. Nutr. 28(6): 399-409 (2004). 2. Drugs R. D. 5(6): 351-354 (2004). 3. Orskov, C., et al., Regul. Pept. 124(1-3): 105-112 (2005). 4. Masui, F., et al., Cell Tissue Res. 318(3): 591-598 (2004). 5. Marti, G., et al., Gene Ther. 11(24): 1780-1785 (2004).