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Keratinocyte Growth Factor, Recombinant Human (KGF, Fibroblast Growth Factor 7, FGF-7)

Cat no: K0201-01A

Keratinocyte Growth Factor, Recombinant Human (KGF, Fibroblast Growth Factor 7, FGF-7)

KGF is a potent specific mitogen for many epithelial cells but not for fibroblasts and endothelial cells. KGF is thought to play an important role in the paracrine growth control of normal epithelial cells. KGF stimulates the proliferation of primary and secondary human keratinocytes to the same extent as EGF.\n\nRecombinant human KGF is a single, non-glycosylated, polypeptide chain containing 164aa and having a molecular weight of 19kD. The sequence of the first five N-terminal aa was determined to be Met-Cys-Asn-Asp-Met. Contains less than 1% dimers and aggregates.\n \nSource: E. coli\n\nMolecular Weight: ~19kD\n\nEndotoxin: 0.1ng/ug \n\nActivity: \nThe ED50 as determined by the dose-dependent stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake) is <10ng/ml, corresponding to a specific activity of 10Units/mg. \n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Stable for at least 6 months at -20 degrees C. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0201-01A

Size

10ug

Applications

ELISA

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile deionized water to a concentration (same/more than)100ug/ml.

Purity

(same/more than)98% (HPLC, FPLC, SDS-PAGE).

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