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Ketoglutarate, alpha BioAssay(TM) Kit

Cat no: K0525

Ketoglutarate, alpha BioAssay(TM) Kit

Ketoglutarate, alpha (a-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing a-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. a-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. \n\nKetoglutarate, alpha BioAssay(TM) Kit provides a simple, sensitive and rapid means for quantifying a-KG in a variety of samples. In the assay, a-KG is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (570nm) and fluorescence (Ex/Em 535/587nm). The Ketoglutarate, alpha BioAssay(TM) Kit is useful for detecting a-KG in the range of 0.01-10nmoles.\n\nKit Components: \nK0525-01: Assay Buffer, 1x25ml. Supplied as a liquid.\nK0525-02: OxiRed Probe, 1x200ul. Supplied as a liquid in DMSO. \nK0525-03. Converting Enzyme, 1x1vial. Supplied as a lypholized powder. \nK0525-04: Development Enzyme Mix, 1x1vial. Supplied as a lypholized powder.\nK0525-05: Standard, 10umol, 1x1vial. Supplied as a lypholized powder.\n\nStorage and Stability:\nStore all components at -20 degrees C. Stable for 2 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

K0525

Size

100Tests

Read more on Supplier website

Applications

ELISA, FC, IF, IHC, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

More info

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

More info

Applications

ELISA, FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse

Conjugates

Biotin

More info
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