KIR2DS4, Fc Chimera, Recombinant, Human (Killer Cell Immunoglobulin-Like Receptor 2DS4, CD158 Antigen-like Family Member I, MHC Class I NK Cell Receptor, Natural Killer-associated Transcript 8, NKAT-8, P58 Natural Killer Cell Receptor Clones CL-39/CL-17,

KIR2DS4 (2DS4), previously called NKAT-8 and designated CD158i, is a type I transmembrane glycoprotein that belongs to the killer cell Ig-like receptor (KIR) family. KIRs are expressed on CD56dim NK cells and T cell subsets where they differentiate normal from abnormal cells and regulate effector functions in the innate immune system. KIRs are named for the number of Ig-like domains (2D or 3D) in the extracellular domain (ECD), and whether they have long or short (L, S) cytoplasmic tails. KIR2DS4 cDNA encodes 304aa including a 21aa signal sequence, a 224aa ECD, a 20aa transmembrane sequence and a 39aa cytoplasmic domain. KIR receptors have no structural orthologs in nonprimates, although mouse 49aa proteins perform similar functions. Like other activating KIRs, KIR2DS4 has a short tail and a positively charged aa within the transmembrane domain that interacts with the ITAM-bearing signaling adaptor, DAP12. Expression of activating KIR is balanced with inhibitory KIR to impart specificity to the immune response. KIR2DS4 recognizes six HLA-C allotypes and two HLA-A allotypes (A*1101 and A*1102) but no HLA-B allotypes. It has also been suggested that KIR2DS4 recognizes a non-MHC ligand, based on specific binding to melanoma cells that lack MHC Class I. A common allele, KIR2DS4*003, is present in up to 89% of Caucasians and ~30% of Asians; it creates a prematurely terminated, soluble, inactive form. Lack of functional KIR2DS4 can potentially create unbalanced inhibition of killer cell function.\n\nSource:\nRecombinant corresponding to aa22-245 from human KIR2DS4 expressed in mouse Murine myeloma cell line, NS0.\n\nMolecular Weight:\n~51.2kD\n\nBiological Activity:\nMeasured by its ability to bind BJAB human Burkitt's lymphoma cells in a flow cytometry assay.\n\nEndotoxin: ~1EU/1ug (LAL)\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 3 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K1858-60A

Size

50ug

Form

Supplied as a lyophilized powder in PBS. BSA free. Reconstitute with PBS to 0.1mg/ml.

Purity

~95% (SDS-PAGE)

References

1. Bottino, C. et al. (1996) Eur. J. Immunol. 26:1816. 2. Lanier, L. L. (2005) Annu. Rev. Immunol. 23:225. 3. Purdy, A.K. and K.S. Campbell (2009) Cancer Biol. Ther. 8:13. 4. Graef, T. et al. (2009) J. Exp. Med. 206:2557. 5. Katz, G. et al. (2001) J. Immunol. 166:7260. 6. Katz, G. et al. (2004) J. Immunol. 173:1819. 7. Maxwell, L.D. et al. (2004) Hum. Immunol. 65:613. 8. Middleton, D. et al. (2007) Hum. Immunol. 68:128.