KIR3DL1, Recombinant, Human (NKB1, nkat3, p70 KIR, Killer Cell Immunoglobulin (Ig)-like Receptor)

The three Ig-domain from of inhibitory killer cell Ig-like receptor 1(KIR3DL1,NKB1, nkat3, p70KIR) is a NK cell receptor for polymorphic HLA-B determinant. KIR3DLl recognizes the Bw4 determinant defined by sequence motifs at positins 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR, which contains two immunoreceptor tyrosine-based inhibition motifs(ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity.\n\nDescription: \nA His-tag fusion protein of KIR3DL1 cytoplasmic tail(361-444aa) was overexpressed as insoluble protein aggregates(inclusion bodies). Recombinant KIR3DL1 produced in E.Coli is a single,non-glycosylated polypeptide chain conjtaining 132 amino acids and having a molecular mass of 15kD.\n\nSequence: \nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHLWCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP\n\nStability: \nStore at 4 degrees C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degrees C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

K1862-29H

Size

5ug

Form

Supplied as a liquid in 25mM Tris-HCl, pH 7.5 and 100mM sodium chloride.

Purity

(same/more than) 95% as determined by RP-HPLC, anion-exchange FPLC and/or reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Contribution of KIR3DL1/3DS1 to ankylosing spondylitis in human leukocyte antigen-B27 Caucasian populations.\n\nArthritis Res Ther 2006 Jun 28;8(4):R101\n\n2. KIR3DL1 polymorphisms that affect NK cell inhibition by HLA-Bw4 ligand.\n\nJ Immunol 2005 Oct 15;175(8):5222-9\n\n3. Interaction between KIR3DL1 and HLA-B*57 supertype alleles influences the progression of HIV-1 infection in a Zambian population.\n\nHum Immunol 2005 Mar;66(3):285-9\n\n4. Crystal structures and KIR3DL1 recognition of three immunodominant viral peptides complexed to HLA-B*2705.\n\nEur J Immunol 2005 Feb;35(2):34 1-51\n\n5. The silent KIR3DP1 gene (CD158c) is transcribed and might encode a secreted receptor in a minority of humans, in whom the KIR3DP1, KIR2DL4 and KIR3DL1/KIR3DS1 genes are duplicated.\n\nEur J Immunol 2005 Jan;35(1):16-24\n\n6. Investigation of killer cell immunoglobulin-like receptor gene diversity: IV. KIR3DL1/S1.\n\nHum Immunol 2004 Jun;65(6):602-12