Kligler Iron Agar (Powder)

Differential medium for identification of enteric bacteria based on fermentation of dextrose, lactose and H2S production. This medium permits differentiation of Gram-negative bacilli by their ability to ferment Dextrose or Lactose, and produce hydrogen sulfide. Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue provide nitrogen, carbon, and vitamins required for organism growth. Ferric Ammonium Citrate and Sodium Thiosulfate are indicators of hydrogen sulfide production. Phenol Red is the pH indicator. Sodium Chloride maintains the osmotic balance of the medium. Agar is the solidifying agent.\n\nFormulation (g/L):\nEnzymatic Digest of Casein 10\nEnzymatic Digest of Animal Tissue 10\nLactose 10\nDextrose 1\nFerric Ammonium Citrate 0.5\nSodium Chloride 5\nSodium Thiosulfate 0.5\nPhenol Red 0.025\nAgar 15\nTotal: 52g/L\n\nDirections per Liter:\n1. Suspend 52g of the medium in one liter of ddH2O.\n2. Heat with frequent agitation and boil for one minute to completely dissolve the medium.\n3. Distribute into test tubes and autoclave for 15 minutes at 121 degrees C.\n4. After autoclaving, allow medium to solidify in a slanted position.\n\nStorage and Stability:\nStore sealed bottle containing the dehydrated medium at RT. Once opened and recapped, place container in a low humidity environment at the same storage temperature. Protect from moisture and light.

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SPECIFICATIONS

Catalog Number

K1892

Size

500g

Form

Light beige, free-flowing homogeneous powder

References

1. Kligler, I. J. 1917. A simple medium for the differentiation of members of the typhoid-paratyphoid group. Am. J. Public Health 7:1042-1044. 2. Russell, F. F. 1911. The isolation of typhoid bacilli from urine and feces with the description of a new double sugar tube medium. J. Med. Res. 25:217. 3. Kligler, I. J. 1918. Modifications of culture media used in the isolation and differentiation of typhoid, dysentery, and allied bacilli. J. Exp. Med. 28:319-322. 4. Bailey, S. F., and L. R. Lacy. 1927. A modification of the Kligler lead acetate medium. J. Bacteriol. 13:183. 5. Sulkin, S. E., and J. C. Willett. 1940. A triple sugar-ferrous sulfate medium for use in identification of enteric organisms. J. Lab. Clin. Med. 25:649-653. 6. Isenberg, H. D. (ed.). 1992. Clinical microbiology procedures handbook, vol.1. American Society for Microbiology, Washington, D.C. 7. Murray, P. R., E. J. Baron, M. A. Pfaller, F. C. Tenover, and R. H. Yolken (eds.). 1995.Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D. C. 8. Bacteriological Analytical Manual. 1995. 8th ed. AOAC International, Gaithersburg, M.D. 9. MacFaddin, J. F. 1985. Media for isolation-cultivation-identification-maintenance of medial bacteria, Williams & Wilkins, Baltimore, MD.