KLK5 BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human KLK5 in cell culture supernates, serum, plasma (heparin, EDTA) human milk and saliva.\n\nKallikrein gene 5 (KLK5, also known as KLK-L2), located on chromosome 19q13.4, is one of the newly identified members of the kallikrein gene family, which is a subgroup of the serine protease enzyme family. In normal human tissues, KLK5 is highly expressed in skin, mammary gland and testis. KLK5 has been suggested to regulate cell shedding (desquamation) in conjunction with KLK7 and KLK14, given its ability to degrade proteins which form the extracellular component of cell junctions in the stratum corneum. It is proposed that KLK5 regulates this process since it is able to self-activate in addition to activating KLK7 and KLK14.\n\nAssay Range:\n78pg/ml-5000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nQ9Y337\n\nEntrez Gene ID:\n25818\n\nKit Components:\n96-well plate pre-coated with anti-KLK5, Human\nLyophilized recombinant KLK5, Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143944

Size

96Tests

References

1. Kim, H., Scorillas, A., Katsaros, D., Yousef, G. M., Massobrio, M., Fracchioli, S., Piccinno, R., Gordini, G., Diamandis, E. P. Human kallikrein gene 5 (KLK5) expression is an indicator of poor prognosis in ovarian cancer. Brit. J. Cancer 84: 643-650, 2001.\n2. Brattsand M, Stefansson K, Lundh C, et al. (2005). \"A proteolytic cascade of kallikreins in the stratum corneum\". J. Invest. Dermatol. 124 (1): 198