KV1.2 Channel Antibody and Membrane Lysate Kit

K_v1.2 Channel Antibody and Membrane Lysate Kit.

K_v1.2 is a mammalian voltage dependent K⁺ channel, homologous to the Drosophilae Shaker K⁺ channel.
K_v1.2 was first cloned from rat brain.
Eight Shaker related genes exist in mammals constituting the K_v1, subfamily of the large K_v channel family of genes.
A functional K_v1 channel is either a membrane spanning homotetramer or heterotetramer, which is composed of members of the same subfamily. In addition several auxiliary subunits and intracellular proteins might interact with the channel and affect its function.
The structure of K_v1.2 channel is similar to all K_v channels and includes six membrane spanning helixes creating a voltage sensor domain and a pore domain.
The channel is expressed in neurons and cardiac and smooth muscle tissue as well as in retina and pancreas.
The crystal structure of K_v1.2 was recently solved shading light on the structure of a mammalian voltage dependent channel.
The functional channel is considered low voltage activated and shows very little inactivation. Therefore, this channel activity influences the membrane potential and excitability of neurons and muscle.
K_v1.2 channels are sensitive to high doses of TEA (560 mM) and low doses of 4-AP (0.59 mM), the пїЅclassicalпїЅ non-selective potassium channel blockers.
Several venomous toxins from Snakes, Scorpions and Honey Bee are potent blockers (affecting the channels in the nanomolar range) of K_v1.2 channels. Among these the most potent and selective are alpha-Dendrotoxin (#D-350, 1-12 nM), Dendrotoxin-I (#D-390, 0.13 nM), Maurotoxin (#STM-340, 0.1-0.8 nM), Hongotoxin-1 (RTH-400, 0.17 nM), Margatoxin (#RTM-325, 0.16-0.65 nM), Tityustoxin Ka (#RTT-360, 0.21 nM) and MCD peptide (#STM-250, 10-400 nM).

Alomone Labs is pleased to offer the K_v1.2 Channel Antibody and Membrane Lysate Kit (#LK-101) which contains a full set of positive and negative controls for western blot analysis.
The kit includes: 0.2 ml of Anti-K_v1.2 antibody (#APC-010), 40 ug of Anti-K_v1.2 negative control antigen, 0.1 ml of K_v1.2 Channel Membrane Lysate (#LX-101) and 0.1 ml of non-injected Xenopus oocyte membrane lysate.

Prices direct from Alomone Labs Ltd.

Quick response times

Exclusive Biosave savings/discounts