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L-Alanine BioAssay(TM) Kit

Cat no: A1205-12

L-Alanine BioAssay(TM) Kit

Alanine is the 2nd most abundant of the 20 proteinogenic amino acids. Nonessential, being\navailable from dietary sources, it plays a key role in the glucose-alanine cycle between tissues and liver. In muscle and other tissues that degrade amino acids, amino groups are pooled as glutamate by transamination. Glutamate then transfers the amino group to pyruvate via alanine aminotransferase, forming alanine and a-ketoglutarate. The alanine is passed into the blood and transported to the liver. A reverse of the alanine aminotransferase reaction takes place in liver. Pyruvate can be used in gluconeogenesis, to form glucose which may return to other tissues through the circulatory system. There appears to be a correlation between alanine levels and and higher blood pressure, energy intake, cholesterol levels, and body mass index. The Alanine Assay Kit provides a sensitive detection method of alanine. In the kit, alanine is converted to pyruvate which is specifically detected leading to proportional color (lambda=570nm: 0-10nmol) or fluorescence (Ex/Em 535/587nm: 0-1nmol) development. Serum concentration: ~24-76ug/ml (~3-9nmol/10ul).\n\nKit Components:\nAlanine *Assay Buffer 1x25ml\nAlanine probe lyophilized\nDMSO 1x0.4ml\nAlanine Converting Enzyme lyophilized\nAlanine Development Mix lyophilized\nAlanine Standard (10umol) lyophilized.\n\nStorage and Stability:\nStore the kit at -20 degrees C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

A1205-12

Size

100Tests

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