L-Asparaginase (Amidohydrolase)

L-Asparaginase is an enzyme that depletes L-Asparagine an important nutrient for cancer cells resulting in cancer/tumor cell starvation. L-asparaginase is an anti-tumor agent derived from E.coli, which can inhibit the growth of malignant cells. It is used mainly for the induction of remission in acute lymphoblastic leukemia. Because of the lymph node origin of malignant B cells in Multiple Myeloma, L-Asparagine is an essential amino acid for their cell metabolism, and, consequently, L-Asparaginase may be of value in managing the disease\nL-Asparaginase produced from E. coli containing 303 amino acids and having a molecular mass of 31.73kD.\n\nUnit Definition: \nOne unit of enzyme catalyzes the hydrolysis of 10 nanomoles of dUTP to dUMP in 1 hour at 85C.\n\nSpecific Activity: ~250U/mg \n\nActivity:\nOne unit of A3800 is defined as the amount of enzyme required to generate 1umol of ammonia per minute at pH 7.3 and 37C.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

A3800

Size

500U

Form

Supplied as a lyophilized powder without additives.

Purity

(same/more than)96% by RP-HPLC and SDS-PAGE.

References

1. Acta Crystallograph. Sect. F Struct. Biol. Cryst. Commun. 61(4): 407-409 (2005). 2. Blood 107: 4244-4249 (2006). 3. Acta Chir. Belg. 105: 667-669 (2005). 4. Hematol. Oncol. 24: 28-32 (2006). 5. Cancer 104: 2858-2861 (2005). 6. Gan To Kagaku Ryoho 32: 1993-1996 (2005).