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L-Carnitine BioAssay(TM) Kit

Cat no: C1385-28

L-Carnitine BioAssay(TM) Kit

Carnitine is a quaternary ammonium compound biosynthesized from the amino acids lysine and methionine. It is required for transport of fatty acids into the mitochondrial matrix via the carnitine/acylcarnitine shuttle where B-oxidation occurs, acetate is generated and the acetate utilized in the TCA cycle for the generation of energy. L-Carnitine is often sold as antioxidant in nutritional supplements. Carnitine exists in two stereoisomers. Only L-carnitine is biologically active. \n\nL-Carnitine BioAssay(TM) Kit is a simple convenient means of measuring free L-Carnitine in biological samples such as serum. The assay transfers an acetyl group from Ac-CoA to carnitine and the free CoA formed is further processed with subsequent oxidation of the Oxi-Red probe to give fluorescence (Ex/Em = 535/587 nm) and absorbance (Lmax = 570 nm). The detection sensitivity for fluorometric assay is ~1uM of L-Carnitine in various samples. Fluorometric assay is ~10X more sensitive than colorimetric assay.\n\nKit Components:\nC1385-28A: Assay Buffer, 1x25ml\nC1385-28B: Probe (in DMSO), 1x200ul\nC1385-28C: Converting Enzyme, 1x1 vial\nC1385-28D: Substrate Mix, 1x400ul\nC1385-28E: Development Mix, 1x1 vial\nC1385-28F: Standard (10umol), 1x1 vial\n\nStorage and Stability:\nStore kit at -20 degrees C. Protect from light. Allow Assay Buffer to warm to room temperature before use. Thaw Probe (in DMSO) in a 37 degrees C water bath prior to use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

C1385-28

Size

100Tests

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