L-Lactate Assay Kit, BioAssay(TM), Colorimetric/Fluorimetric

Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology.\n\nSimple, direct and automation-ready procedures for measuring lactate concentration are very desirable. Lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at lex/em=530/585 nm, is proportional to the lactate concentration in the sample.\n\nApplications:\nDirect Assays: L-lactate in serum, plasma, urine, cell media samples and other biological samples.\n\nKey Features:\nSensitive and accurate. Detection limit of 1uM and linearity up to 50uM L-lactate in 96-well plate assay.\nConvenient. The procedure involves adding a single working reagent, and reading the fluorescence after 60 min. Room temperature assay.\nHigh-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.\n\nKit Contents:\nAssay Buffer: 10ml Enzyme A: 120ul\nNAD Solution: 1ml Enzyme B: 120ul\nProbe: 750ul Standard: 1ml\nStorage conditions. Store all reagents at -20 degrees C. Shelf life: 6 months after receipt.\nPrecautions: reagents are for research use only. Normal precautions for laboratory reagents should be exercised while using the reagents. Please refer to Material Safety Data Sheet for detailed information.\n\nSample Preparation And Considerations:\nThe following substances interfere and should be avoided in sample preparation: EDTA (>0.5mM), ascorbic acid, SDS (>0.2%), sodium azide, NP-40 (>1%) and Tween-20 (>1%). Serum and plasma samples should be diluted at least 200

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SPECIFICATIONS

Catalog Number

L1011-04B

Size

1Kit

References

1. Senadheera D et al (2009). Inactivation of VicK affects acid production and acid survival of Streptococcus mutans. J Bacteriol. 191(20): 6415- 24.\n2. Le Nihouannen D et al (2009). Ascorbic acid accelerates osteoclast formation and death. Bone 46(5): 1336-43.\n3. Milovanova TN et al (2008). Lactate stimulates vasculogenic stem cells via the thioredoxin system and engages an autocrine activation loop involving hypoxia-inducible factor 1. Mol Cell Biol. 28(20): 6248-61.