La/SS-B Antigen, Rabbit Thymus

The La/SS-B antigen is a 47kD ribonucleic protein that is associated with a spectrum of small RNAs (2). La/SS-B appears to be readily susceptible to proteolysis, resulting in many smaller (42, 320, and 270kD) but still immunoreactive polypeptides (3). It has been shown that the La/SS-B antigen primarily resides in the nucleus (2). It appears to be associated with RNA polymerase III transcripts (4). It has also been shown that the La/SS-B antigen shows strong conservation across species (5). Anti-La/SS-B autoantibodies were described originally as precipitating autoantibodies in sera of Sjogren's Syndrome patients and referred to as SjT.(1) A precipitin with the same properties was rediscovered later and termed La and then SS-B. Anti-La/SS-B precipitins.The presence of autoantibodies against the La/SS-B antigen has been advocated as a diagnostic aid in Sjogren's Syndrome patients (6). Autoantibodies against La/SS-B are also commonly found in Systemic Lupus Erythematosus and Subacute Cutaneous Lupus (4). In addition, there appears to be a correlation between anti-La/SS-B and the absence of nephritis in SLE patients. Studies have demonstrated that SLE patients with precipitating Anti-Ro/SS-A antibodies have a high incidence of serious nephritis (53%), while those with both Anti-Ro/SS-A and Anti-La/SS-B have a low (9%) frequency of nephritis (7).\n\nActivity:\nReacts with La/SS-B antisera in immunodiffusion. No reaction is observed with antisera to nRNP, Ro/SS-A, Sm, Scl-70, or Jo-1 in immunodiffusion. The activity of this product will vary with assay conditions.\n\nReconstitution:\nReconstitute with 1ml cold distilled water (sterile). Some precipitate may occur but will not affect product performance. Do not store diluted antigen.\n\nStorage and Stability: \nAliquot to avoid repeated freezing and thawing and freeze at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.

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SPECIFICATIONS

Catalog Number

L1380-06

Size

5mg

Applications

ELISA, WB

Form

Supplied as a lyophilized powder in 0.01M phosphate buffer, 0.13M sodium chloride, 0.02% sodium azide.

Purity

Purified by a combination of protein fractionation and column chromatography. Most contaminating antigens have been removed by immunoadsorbent methodology.