La/SS-B Antigen, Recombinant, Human

LA/SS-B (SS-B Antigen) is a cytoplasmic or nuclear ribonucleoproteins carrying LA determinant. SS-B/La is a nuclear protein of 48kD with two structural domains of Mr 28,000 and Mr 23,000 generated by proteolytic cleavage.The human La (SS-B) autoantigen is an abundantly expressed putative RNA chaperone, functioning in various intracellular processes involving RNA.\n\nRecombinant Human LA/SS-B is a single, non-glycosylated, polypeptide chain having a molecular mass of 47.6kD.\n\nDimers and Aggregates:\n(same/less than) 1% as determined by silver-stained SDS-PAGE gel analysis.\n\nEndotoxin:\n(same/less than) 0.1ng/ug (IEU/ug) of LA/SS-B\n\nProtein Content:\nProtein quantitation was carried out by using:\n0.25-2.0mg/ml Bradford assay vs. BSA\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1380-07

Size

5ug

Form

Supplied as a liquid in 10mM sodium phosphate, pH 6.0, 150mM sodium chloride and 0.05% sodium azide.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

1. Expression of Ro/SS-A and La/SS-B determined by immunohistochemistry in healthy, inflamed and autoimmune diseased human tissues: a generalized phenomenon. van Woerkom JM, Geertzema JG, Kruize AA, Clin Exp Rheumatol 2004 May-Jun;22(3):285-92 2. The human La (SS-B) autoantigen interacts with DDX15/hPrp43, a putative DEAH-box RNA helicase. Fouraux MA, Kolkman MJ, De Jong AS, RNA 2002 Nov;8(11):1428-43 3. Antibodies to Ro/SS-A and La/SS-B in systemic lupus erythematosus and other autoimmune disorders. Pradhan VD, Badakere SS, Dolas MP, J Assoc Physicians India 2002 Jun;50:762-5 4. Restricted specificity of intermolecular spreading to endogenous La (SS-B) and 60 kDa Ro (SS-A) in experimental autoimmunity. Gordon TP, Kinoshita G, Keech C, Purcell A, Scand J Immunol 2002 Aug;56(2):168-73 5. Detailed analysis of the phosphorylation of the human La (SS-B) autoantigen. (De)phosphorylation does not affect its subcellular distribution. Broekhuis CH, Neubauer G, Mann M, Biochemistry 2000 Mar 21;39(11):3023-33 6. The La (SS-B) autoantigen, a key protein in RNA biogenesis, is dephosphorylated and cleaved early during apoptosis. Rutjes SA, Utz PJ, Broekhuis C, Cell Death Differ 1999 Oct;6(10):976-86.