Lactate Dehydrogenase B, Recombinant (LDHB, LDH-B, L-Lactate dehydrogenase B chain)

A tetrameric enzyme that, along with the coenzyme NAD+, catalyzes the interconversion of lactate and pyruvate. In vertebrates, genes for three different subunits (LDH-A, LDH-B and LDH-C) exist. Recombinant LDH-B was produced using LDH-B DNA cloned from cDNA library of chicken heart. \n\nBiological Activity: ~250 U/mg protein\n\nUnit Definition:\nOne unit is defined as 1 micromole of NAD+ produced per minute under the assay conditions (25 degrees C, pH 7.5). Both transaminase activities include alpha-hydroxyglutarate dehydrogenase activity.\n\nStorage and Stability:\nStore lyophilized powder dessicated at -20 degrees C. Upon reconstitution, product may be stored at 4 degrees C for 2-7 days and at -20 degrees C for long-term storage. Prevent freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L1011-08

Size

1mg

Form

Supplied as a lyophilized powder in potassium phosphate. Reconstitute with sterile ddH2O to (same/more than)100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 95% by RP-HPLC and SDS-PAGE. Chromatographically purified. Endotoxin: (same/less than) 0.1ng/ug (IEU/ug)

References

1. Effect of filgrastim on serum lactate dehydrogenase and alkaline phosphatase values in early breast cancer patients. Papaldo P, et al., Cancer Invest 2004;22(4):650-3 2. Chaperone-like manner of human neuronal tau towards lactate dehydrogenase. Tian R, Nie CL, Neurochem Res 2004 Oct;29(10):1863-72 3. Molecular characterization of two lactate dehydrogenase genes with a novel structural organization on the genome of Lactobacillus sp. strain MONT 4. Weekes J, Yuksel GU, Appl Environ Microbiol 2004 Oct;70(10):6290-5 4. Roche IFCC methods for lactate dehydrogenase tested for duplicate errors with Greiner and Becton-Dickinson lithium-heparin and Greiner serum samples. Dimeski G, et al. Clin Chem 2004 Dec;50(12):2391-2 5. The application and mechanisms of polyethylene glycol 8000 on stabilizing lactate dehydrogenase during lyophilization. Mi Y, Wood G, PDA J Pharm Sci Technol 2004 Jul-Aug;58(4):192-202 6. Persistent histidine-rich protein 2, parasite lactate dehydrogenase, and panmalarial antigen reactivity after clearance of Plasmodium falciparum monoinfection. Iqbal J, et al., J Clin Microbiol 2004 Sep;42(9):4237-41.