Lactate Dehydrogenase, Chicken Heart (LDH CH, LD, LDHA, LDHB, LDHH, LDH1, LDH Muscle Subunit, LDHM, NY-REN-46 REN-59 Antigens, Proliferation Inducing Gene 19 Protein, PIG19, TRG5)

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36kD subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H)-in the heart, LDH-2 (3H1M)-in the reticuloendothelial system, LDH-3 (2H2M)-in the lungs, LDH-4 (1H3M)-in the kidneys and LDH-5 (4M) in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a flipped pattern) suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization. \n\nSpecifications\nL-Lactate: NAD+ oxidoreductase (EC 1.1.1.27) from chicken heart\n\nSpecific Activity: (same/more than) 200u/mg protein (at 25 degrees C and pH 7.0)\n\nContaminants: (as LDH activity = 100%) \nMalate dehydrogenase (same/less than) 0.03% \nMyokinase (same/less than) 0.01% \nPyruvate kinase (same/less than) 0.01% \nGlutamic-pyruvic transaminase (same/less than) 0.03% \nGlutamic-oxaloacetic transaminase (same/less than) 0.03%\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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