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Lactate Dehydrogenase (LDH)

Cat no: 143071

Lactate Dehydrogenase (LDH)

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36kD subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H)-in the heart, LDH-2 (3H1M)-in the reticuloendothelial system, LDH-3 (2H2M)-in the lungs, LDH-4 (1H3M)-in the kidneys and LDH-5 (4M) in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a flipped pattern) suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.\n\nSource:\nHuman Erythrocytes\n\nUnit Definition:\nOne unit will catalyze the oxidation of one micromole of L-lactate to pyruvate/minute at 37C and pH 8.55.\n\nIsoenzyme Electrophoresis:\nLD1: 37%\nLD2: 26%\nLD3: 29%\nLD4: 4%\nLD5: 4%\n\nStorage and Stability:\nMay be stored at 4 degrees C. For long-term storage, aliquot and store at 4 degrees C. Do not freeze. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

143071

Size

100U

Form

Supplied as a liquid in 20mM Tris-Chloride, 3.1M Ammonium Sulfate, 1mM DTT, 1mM EDTA, pH 7.5. No preservative added.

Purity

Purified

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