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Lactate Dehydrogenase (LDH)

Cat no: 143072

Lactate Dehydrogenase (LDH)

Lactate dehydrogenase is also known as L-lactate dehydrogenase A chain, LDH-A, LDH muscle subunit and LDH-M. Two isozymes of LDH occur in mammals, LDH-M and LDH-H which come together to form a homotetramer of 36kD subunits. Every LDH molecule consists of four subunits, where each subunit is either H each M (based on their electrophoretic properties.) There are, therefore, five LDH isotypes: LDH-1 (4H)-in the heart, LDH-2 (3H1M)-in the reticuloendothelial system, LDH-3 (2H2M)-in the lungs, LDH-4 (1H3M)-in the kidneys and LDH-5 (4M) in the liver and striated muscle. Usually LDH-2 is the predominant form in the serum. An LDH-1 level higher than the LDH-2 level (a flipped pattern) suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH-1, into the bloodstream). In general, LDH is often used as a marker of tissue breakdown. LDH shows a cytoplasmic localization.\n\nSource:\nHuman heart\n\nUnit Definition: \nOne unit will catalyze the oxidation of one micromole of L-lactate to pyruvate/minute at 37C and pH 8.55.\n\nIsoenzyme Electrophoresis:\nLD1: 71%\nLD2: 17%\nLD3: 5%\nLD4: 3%\nLD5: 4%\n\nContaminants:\nCK: <0.1%\nGOT: <0.1%\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

143072

Size

100U

Form

Supplied as a lyophilized powder in 20mM Tris0chloride, 1mM DTT, 1mM EDTA, pH 7.5. No Preservative added.

Purity

Purified

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