Lactate Dehydrogenase (LDH) Cytotoxicity Assay Kit, BioAssay(TM)

Lactate Dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme that upon membrane damage is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage. The colorimetric LDH release assay is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of the tetrazolium salt MTT to a formazan dye which exhibits an absorption maximum at 565 nm.\n\nKey Features:\nSafe. Non-radioactive assay (cf. chromium release assay).\nFast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.\nHomogeneous and convenient. "Mix-incubate-measure" type assay. No wash steps are involved.\nRobust and amenable to HTS. Can be readily automated with HTS liquid handling systems.\n\nApplications:\nCytotoxicity and Apoptosis: evaluation of toxic compounds, toxins, detergents, environmental pollutants etc.\nDrug Discovery: high-throughput screen for drug toxicity.\n\nKit Contents: (100 Tests in 96-Well Plate)\nSubstrate Buffer, 1x20ml \nPMS Reagent, 1x1.5ml\nNAD Solution, 1x1ml \n10% Triton X-100, 1x1ml\nMTT Reagent, 1x1.5ml\n\nStorage and Stability:\nStore components at -20 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L1013-98

Size

1Kit

References

1. Korzeniewski C. and Callewaert DM. (1983). An enzyme-release assay for natural cytotoxicity. J Immunol Methods 64(3): 313-320.\n2. Weidmann E. et al. (1995). Lactate dehydrogenase-release assay: A reliable, nonradioactive technique for analysis of cytotoxic lymphocyte- mediated lytic activity against blasts from acute myelocytic leukemia. Ann Hematol 70(3): 153-158.\n3. Kawai et al. (1992). Additive effects of antitumor drugs and lymphokine- activated killer cell cytotoxic activity in tumor cell killing determined by lactate-dehydrogenase-release assay. Cancer Immunol Immunother 35(4): 225-229.