Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Lactate Dehydrogenase (LDH) Cytotoxicity BioAssay(TM) Kit provides a fast and simple method for quantitating cytotoxicity based on the measurement of activity of lactate dehydrogenase (LDH) released from damaged cells. Unlike many other cytoplasmic enzymes which exist in many cells either in low amount (e.g., alkaline and acid phosphatase) or unstable, LDH is a stable cytoplasmic enzyme present in all cells and rapidly released into the cell culture supernatant upon damage of the plasma membrane. LDH activity can be determined by a coupled enzymatic reaction: LDH oxidizes lactate to pyruvate which then reacts with tetrazolium salt INT to form formazan. The increase in the amount of formazan produced in culture supernatant directly correlates to the increase in the number of lysed cells. The formazan dye is water-soluble and can be detected by spectrophotometer at 500nm. The LDH Cytotoxicity BioAssay is sensitive, convenient, precise, and is applicable to a variety of cytotoxicity studies.
Assay Time: ~0.5-1 hr.
Kit Components:
L1014-01A: Catalyst, 1x1vial
L1014-01B: Dye Solution , 1x45ml
Storage and Stability:
Store kit components at -20 degrees C. Kit components are stable for 6-12 months.
The prepared Catalyst Solution, Dye Solution and Reaction mixture are stable for several weeks at 4 degrees C.
Quick Search
Products 
