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Lactate Dehydrogenase (LDH) Kit, BioAssay(TM)

Cat no: L1013-95

Lactate Dehydrogenase (LDH) Kit, BioAssay(TM)

Lactate Dehydrogenase (LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. When disease or injury affects tissues containing LDH, the cells release LDH into the bloodstream, where it is identified in higher than normal levels. Therefore, LDH is most often measured to evaluate the presence of tissue or cell damage. The non-radioactive colorimetric LDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The intensity of the purple color formed is directly proportional to the enzyme activity.\n\nKey Features:\nHigh sensitivity and wide linear range. Use 3ul serum or plasma sample. The detection limit is 2 IU/L, linear up to 200 IU/L. \nHomogeneous and simple procedure. Simple

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SPECIFICATIONS

Catalog Number

L1013-95

Size

1Kit

References

1. Stentz R., et al. (2010). Controlled protein release from viable Lactococcus lactis cells. Appl. Environ. Microbiol. 76(9): 3026-3031.\n2. Lu F., et al. (2008). The effect of C1 inhibitor on intestinal ischemia and reperfusion injury. Am. J. Physiol. Gastrointest. Liver Physiol. 295(5): G1042-G1049.\n3. Mittal R., et al. (2009). Brain damage in newborn rat model of meningitis by Enterobacter sakazakii: a role for outer membrane protein A. Lab. Invest. 89(3): 263-277.

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