Laminin, Mouse

Laminin is a major component of basement membranes and stimulates neurite outgrowth, promotes cell attachment, chemotaxis and cell differentation. Promotes the adhesion and growth of human carcinoma and sarcoma cells, human retinoblastoma cells, liver cells, mouse neuroblastoma cells, mouse embryonal carcinoma cells, and seems to be involved in the development of embryonal tissue.\n\nApplications:\nSuitable for use in ELISA, production of antiserum, cell adhesion assays, and neurite-stimulation assays. Active in cell attachment.\n\nRecommended Dilution:\n1-10ug/cm2 of growth surface depending on cell type.\nThe optimal laminin concentration may vary with the cell type and has to be determined experimentally.\n\nMW: 900kD (unreduced)\n\nQuality Control:\nTested for ability to initiate differentiation (neurite outgrowth) of NG-108 human neuroblastoma cells.\n\nStorage and Handling: \nStore at -20 degrees C. Thaw carefully at RT and do not refreeze! Store thawed material at 2

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SPECIFICATIONS

Catalog Number

L1225-08A

Size

1mg

Applications

ELISA

Form

Supplied as a liquid in 0.05M Tris HCl, 0.15M sodium chloride, pH 7.4. No preservative added. Sterile-filtered and tested negative for bacteria, fungi and mycoplasma.

Purity

(same/more than) 90% by SDS-PAGE. Contains entactin.