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Lck, p56lck, active, Recombinant, Human (Lymphocyte-specific Tyrosine Kinase)

Cat no: L1565-06C

Lck, p56lck, active, Recombinant, Human (Lymphocyte-specific Tyrosine Kinase)

LCK (p56lck) is a member of the Src family of non-receptor tyrosine kinases. It was identified as a gene rearranged and over-expressed in the murine lymphoma LSTRA, most likely as a result of the insertion of Moloney murine leukemia virus DNA immediately adjacent to the gene (1). LCK behaves as a proto-oncogene and can lead to cell transformation upon activation. A number of human cancer cell lines show over-expression of LCK, pointing to a possible role for this kinase in the initiation and maintenance of the transformed state in human cancers (2).\n\nSource: Recombinant full-length human LCK was expressed by baculovirus in Sf 9 insect cells using an N-terminal GST tag. The gene accession number is NM_005356.\n\nMolecular Mass: The approximate molecular weight is 84 kD.\n\nActivity: The specific activity of LCK was determined to be 239 nmol/min/mg using a Poly (Glu:Tyr, 4:1) synthetic peptide substrate (see activity assay protocol).\n\nStorage: This product is stable at (same/less than) -70 degrees C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

L1565-06C

Size

10ug

Form

Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, 25% glycerol.

Purity

(same/more than) 90% by densitometry

References

1. Fischer, S. et al. (1987) Biochem. Biophys. Res. Commun. 143:819. \n2. Veillette, A. et al. (1987) Oncogene Res. 1:357.

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