Catalogue number: |
505011 |
Reactivities: |
Mouse |
Hosts: |
Rat |
Applications: |
Flow Cytometry, Immunocytochemistry, Immunohistochemistry |
Size: |
50 microg |
Clone: |
JES5-16E3 |
Gene: |
16153 |
Dilutions: |
Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 4-8 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of IL-10 recombinant protein ranging from 2000 to 15 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application. * For ELISA/ELISPOT capture, it is very critical to use 0.2 M Sodium Phosphate Buffer, pH 6.5 (11.8g Na2HPO4, 16.1g NaH2PO4; q.s. to 1.0 L) as coating buffer.Note: Carbonate buffer, pH 9.5 should not be used as coating buffer for JES5-2A5. It may cause high background and lower sensitivity. |
Storage buffer: |
The antibody solution should be stored undiluted at 4C. This LEAF(TM) solution contains no preservative; handle under aseptic conditions. |
Concentration: |
1 mg/ml |
Antigen: |
E. coli-expressed, recombinant mouse IL-10 |
Isotype: |
Rat IgG2b, kappa |
Clonality: |
Monoclonal |
Regulatory status: |
RUO |
Shipping temp: |
Ambient RT |
Storage temp: |
4 C |
Alternative names: |
Interleukin-10 (IL10), CSIF, B-TCGF, BTCGF, TGIF |