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LEAF(TM) Purified anti-mouse/rat TNF-alpha

LEAF(TM) Purified anti-mouse/rat TNF-alpha

Cat no: 506105


Supplier: BioLegend
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TNF-alpha is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-alpha. Monomeric mouse TNF-alpha is 156 amino acid protein (N-glycosylated) with a reported molecular weight of 17.5 kD protein. TNF-alpha forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-alpha has also been described. TNF-alpha binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils. The TN3-19.12 antibody reacts with mouse, rat, and rabbit tumor-necrosis factor-alpha (TNF-alpha) proteins. The TN3-19.12 antibody can neutralize the bioactivity of natural or recombinant TNF-alpha.
Catalogue number: 506105
Reactivities: Mouse, Rat
Hosts: Hamster
Applications: Flow Cytometry, Immunocytochemistry, Immunoprecipitation, Western Blot
Size: 50 microg
Clone: TN3-19.12
Gene: 21926,24835
Dilutions: Each lot of this antibody is quality control tested by ELISA assay. For ELISA capture applications, a concentration range of 2-6 microg/ml is recommended. To obtain a linear standard curve, serial dilutions of mouse TNF-alpha recombinant protein ranging from 500 to 4 pg/ml are recommended for each ELISA plate. It is recommended that the reagent be titrated for optimal performance for each application.
Storage buffer: The antibody solution should be stored undiluted at 4C. This LEAF(TM) solution contains no preservative; handle under aseptic conditions.
Concentration: 1 mg/ml
Antigen: E. coli-expressed, recombinant mouse TNF-alpha
Isotype: Armenian Hamster IgG
Clonality: Monoclonal
Regulatory status: RUO
Shipping temp: Ambient RT
Storage temp: 4 C
Alternative names: Tumor necrosis factor-alpha, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
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