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Products LEAF(TM) Purified anti-rat IFN-gamma
| Catalogue number: | 507808 |
| Reactivities: | Mouse, Rat |
| Hosts: | Mouse |
| Applications: | Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Western Blot |
| Size: | 500 microg |
| Clone: | DB-1 |
| Gene: | 25712 |
| Dilutions: | Each lot of this antibody is quality control tested by ELISA analysis. For ELISA capture applications, the suggested use of this reagent is 1-4 ug/ml. For use as an ELISPOT capture antibody, the range of 2-6 ug/ml is recommended.To obtain a linear standard curve, serial dilutions of IFN-gamma recombinant protein ranging from 1000 to 8 pg/ml are recommended for each ELISA plate. The Purified DB-1 has been tested by blocking fluorochrome conjugated DB-1 for intracellular cytokine staining. In order to obtain complete blocking results, a saturated amount of Purified antibody (? 5.0 ug/million cells) should be used for incubation with target cells, prior to staining with fluorochrome conjugated antibody. It is recommended that the reagent be titrated for optimal performance for each application. |
| Storage buffer: | The antibody solution should be stored undiluted at 4C. This LEAF(TM) solution contains no preservative; handle under aseptic conditions. |
| Concentration: | 1 mg/ml |
| Antigen: | Recombinant rat IFN-gamma |
| Isotype: | Mouse IgG1, kappa |
| Clonality: | Monoclonal |
| Regulatory status: | RUO |
| Shipping temp: | Ambient RT |
| Storage temp: | 4 C |
| Alternative names: | Interferon-gamma, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF) |
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