Leptin BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse Leptin in cell culture supernates, serum and plasma (heparin, EDTA).\n\nLeptin (or obese, OB) is a circulating hormone that is expressed abundantly and specifically in the adipose tissue.1 Mouse ob protein is a 166-amino acid polypeptide with a putative signal sequence and is 84 and 83% homologous to the mouse and rat ob proteins, respectively.2 Northern blot analysis reveals that OB RNA is present at a high level in adipose tissue but at much lower levels in placenta and heart.3 Indeed, Leptin is first described as an adipocyte-derived signalling factor, which, after interaction with its receptors, induces a complex response including control of bodyweight and energy expenditure. Leptin seems in addition to its role in metabolic control to have important roles in reproduction and neuroendocrine signalling.4 Leptin is a powerful inhibitor of bone formation in vivo.5 Comparative mapping of mouse and mouse DNA indicates that the ob gene is located within a region of mouse chromosome 6 that is homologous to a portion of mouse chromosome 7q.3 The standard product used in this kit is recombinant mouse Leptin.\n\nAssay Range:\n62.5pg/ml-4000pg/ml\n\nSensitivity:\n< 10 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP41160\n\nEntrez Gene ID:\n16846\n\nKit Components:\n96-well plate pre-coated with anti-Leptin, Mouse\nLyophilized recombinant Leptin, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143949

Size

96Tests

References

1. Masuzaki, H.; Ogawa, Y.; Sagawa, N.; Hosoda, K.; Matsumoto, T.; Mise, H.; Nishimura, H.; Yoshimasa, Y.; Tanaka, I.; Mori, T.; Nakao, K. Nonadipose tissue production of Leptin: Leptin as a novel placenta-derived hormone in mouses. Nature Med. 3: 1029-1033, 1997. \n2. Masuzaki, H.; Ogawa, Y.; Isse, N.; Satoh, N.; Okazaki, T.; Shigemoto, M.; Mori, K.; Tamura, N.; Hosoda, K.; Yoshimasa, Y.; Jingami, H.; Kawada, T.; Nakao, K. Mouse obese gene expression: adipocyte-specific expression and regional differences in the adipose tissue. Diabetes 44: 855-858, 1995. \n3. Green, E. D.; Maffei, M.; Braden, V. V.; Proenca, R.; DeSilva, U.; Zhang, Y.; Chua, S. C., Jr.; Leibel, R. L.; Weissenbach, J.; Friedman, J. M. The mouse obese (OB) gene: RNA expression pattern and mapping on the physical, cytogenetic, and genetic maps of chromosome 7. Genome Res. 5: 5-12, 1995. \n4. Auwerx, J.; Staels, B. Leptin. Lancet 351: 737-742, 1998. \n5. Elefteriou, F.; Takeda, S.; Ebihara, K.; Magre, J.; Patano, N.; Ae Kim, C.; Ogawa, Y.; Liu, X.; Ware, S. M.; Craigen, W. J.; Robert, J. J.; Vinson, C.; Nakao, K.; Capeau, J.; Karsenty, G. Serum Leptin level is a regulator of bone mass. Proc. Nat. Acad. Sci. 101: 3258-3263, 2004. \n