Leptin Receptor B, Mouse, phosphorylated Control Peptide (Tyr1138) (ObRb, Obese Receptor B)

Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (146 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LEPR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894aa). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling. Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb.\n\nSource:\nAn 11 amino acid sequence aa1133-1143 within the cytoplasmic C-terminus of mouse OBR. \nMouse sequence is specific for the long isoform (OBRb). Species Sequence Homology: rat 91% ; human 64% \nApplications:\nSuitable for use in Antibody Blocking and ELISA. Not suitable for Western Blot. Other applications not tested. \n\nRecommended Dilution:\nAntibody Blocking: 5-10ug/1ug of affinity purified IgG or 1ul antiserum.\nELISA: 10-100ng/well\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1671-13A

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.5

Purity

Highly purified

References

1. Bjorbaek C et al (1999) J. Biol. Chem. 274, 30059; 2. Bjorbaek C et al (2000) J. Biol. Chem. 275, 40649; 3. Bank AS et al (2000) J. Biol. Chem. 275, 14563; 4. Bjorbaek C et al (1998) Mol. Cell 1, 619; 5. Tartaglia, LA (1995) Cell 83, 1263; 6. Chen H et al (1996) Cell 84, 491-495; 7. Lee GH et al (1996) Nature 379, 632-635; 8. Cioffi JA et al (1996) Nat. Med. 2, 585-589