Leptin receptor BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse Leptin receptor in cell culture supernates, serum and plasma (heparin, EDTA).\n\nLeptin receptor (or Obese receptor, OBR) is a single membrane-spanning receptor most related to the gp130 signal-transducing component of the IL-6 receptor, the G-CSF receptor, and the LIF receptor. OB-R mRNA is expressed not only in choroid plexus, but also in several other tissues, including hypothalamus.1 Leptin acts through the leptin receptor, a single-transmembrane domain receptor of the cytokine-receptor family.2 Leptin controls energy balance and food intake through the leptin receptor in the hypothalamus of the brain, which suggests that some polymorphisms of the leptin receptor gene (LEPR) might contribute to obesity or obesity-related diseases.3 Leptin is also involved in the regulation of blood pressure through the leptin receptor.4 \n\nAssay Range:\n156pg/ml-10,000pg/ml\n\nSensitivity:\n< 5 pg/ml\n\nCrossreactivity:\nThere is no detectable cross-reactivity with other relevant proteins.\n\nUniprot ID:\nP48356\n\nEntrez Gene ID:\n16847\n\nKit Components:\n96-well plate pre-coated with anti-Leptin receptor, Mouse\nLyophilized recombinant Leptin receptor, Mouse standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143951

Size

96Tests

References

1. Tartaglia, L. A.; Dembski, M.; Weng, X.; Deng, N.; Culpepper, J.; Devos, R.; Richards, G. J.; Campfield, L. A.; Clark, F. T.; Deeds, J.; Muir, C.; Sanker, S.; Moriarty, A.; Moore, K. J.; Smutko, J. S.; Mays, G. G.; Woolf, E. A.; Monroe, C. A.; Tepper, R. I. Identification and expression cloning of a leptin receptor, OB-R. Cell 83: 1263-1271, 1995. \n2. Clement, K.; Vaisse, C.; Lahlou, N.; Cabrol, S.; Pelloux, V.; Cassuto, D.; Gourmelen, M.; Dina, C.; Chambaz, J.; Lacorte, J.-M.; Basdevant, A.; Bougneres, P.; Lebouc, Y.; Froguel, P.; Guy-Grand, B. A mutation in the human leptin receptor gene causes obesity and pituitary dysfunction. Nature 392: 398-401, 1998.\n3. Park, K. S.; Shin, H. D.; Park, B. L.; Cheong, H. S.; Cho, Y. M.; Lee, H. K.; Lee, J.-Y.; Lee, J.-K.; Oh, B.; Kimm, K. Polymorphisms in the leptin receptor (LEPR)--putative association with obesity and T2DM. J. Hum. Genet. 51: 85-91, 2006.\n4. Rosmond, R.; Chagnon, Y. C.; Holm, G.; Chagnon, M.; Perusse, L.; Lindell, K.; Carlsson, B.; Bouchard, C.; Bjorntorp, P. Hypertension in obesity and the leptin receptor gene locus. J. Clin. Endocr. Metab. 85: 3126-3131, 2000. \n