Leptin Receptor, Mouse, Control Peptide (OBR, LEPR, B219, Obese Receptor)

Control Peptide for L1671-09A (antiserum) and L1671-09 (affinity purified)\n\nA 15 amino acid sequence near the N-terminus (extracellular domain) of mouse OBR was selected for antibody production .\n\nObesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (146 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LEPR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894aa). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling. Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb. The absence of functional leptin in ob/ob and the long form OBRb in db/db mice due to abnormal splicing produces severe obesity. The nature of leptin resistance in other obesity models is not clear.\n\nApplications:\nSuitable for use in Antibody Blocking and ELISA. Not suitable for Western Blot. Other applications not tested. \n\nRecommended Dilution:\nAntibody Blocking: 5-10ug/1ug of affinity purified IgG or 1ul antiserum.\nELISA: 10-100ng/well\nOptimal dilutions to be determined by the researcher. \n\nStorage and Stability\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1671-09B

Size

50ug

Applications

ELISA, WB

Reactivities

Mouse

Form

Supplied as a liquid in PBS, pH 7.2

Purity

Highly purified

References

1. Tartaglia, LA (1995) Cell 83, 1263-1271; 2. Chen, H (1995) Cell 84, 491-495; 3. Lee, G-H, et al (1996) Nature 379, 632-6352; 4. Cioffi JA et al (1996) Nature Med. 2, 585-589; 5. Takya K et al (1996) BBRC 225, 75-83; 6. Considine,R.V et al (1996) Diabetes 45, 992-994; 7. Considine,R.V et al (1997) BBRC 233, 248-252

Additional Info

Mouse sequence is common in all six alternatively spliced mouse/rat obese receptors (a-e forms