Leptin Receptor, Mouse (OBR, LEPR, B219, Obese Receptor) (Control Peptide)

Control Peptide for L1671-11A (antiserum) and L1671-11 (affinity purified) antibodies.\n\nObesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (146 amino acid, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin receptor or Obese receptors (OBR or LEPR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. The OBRa represents the initially identified mouse Ob-R (short form, 894aa). OBRa, -c, -d, and -f differ in sequence after Lys889 and have short (30-40 aa) cytoplasmic extension Mouse Ob-Rb (long form) displays ~78% homology to the human Ob-R (long form, 1165aa). OBRb has ~300 aa intracellular tail. Expression of Ob-Rb and other forms have been detected in hypothalamus and other tissues. OBRe lacks the transmembrane domain. The soluble Ob-Re is found in adipose tissues, hypothalamus, heart, and testes. Ob-R is abnormally spliced in db/db mice (truncation of cytoplasmic domain) that are important for leptin signaling. Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb. The absence of functional leptin in ob/ob and the long form OBRb in db/db mice due to abnormal splicing produces severe obesity. \n\nSpecificity:\nAn 18 amino acid sequence near the C-terminus of mouse OBRb (long form). Unique to the OBRb long form. It is not found in OBR isoforms a and c-f. \n\nSpecies sequence homology: \nIt is 94% conserved rat, 88% in monkey, human and 77% in porcine OBRb. No significant homology to gp130. \n\nApplications:\nSuitable for use in ELISA and Antibody Blocking. Not suitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 50-100ng control peptide/well.\nAntibody Blocking: 5-10ug per 1ug L1671-11 (affinity purified) or per 1ul L1671-11A (antiserum).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1671-11B

Size

50ug

Applications

ELISA, WB

Form

Supplied as a liquid in PBS, pH 7.2

Purity

Highly purified

References

Tartaglia, LA (1995) Cell 83, 1263-1271; Chen, H (1995) Cell 84, 491-495; Lee, G-H, et al (1996) Nature 379, 632-6352; Cioffi JA et al (1996) Nature Med. 2, 585-589; Takya K et al (1996) BBRC 225, 75-83; Considine,R.V et al (1996) Diabetes 45, 992-994; Considine,R.V et al (1997) BBRC 233, 248-252\n