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Leptin, Recombinant, Chicken (Obesity Factor, OB, LEP)

Cat no: 136920

Leptin, Recombinant, Chicken (Obesity Factor, OB, LEP)

Leptin is a protein hormone with important effects in regulating body weight, metabolism and reproductive function. The protein is approximately~16kD in mass and encoded by the obese (ob)gene. leptin is expressed predominantly by adipocytes, which fits with the idea that body weight is sensed as the total mass of fat in the body. Smaller amounts of leptin are also secreted by cellsin the epithelium of the stomach and in the placenta. Leptin receptors are highly expressed in areas of the hypothalamus known to be important in regulating body weight, as well as in T lymphocytes and vascular endothelial cells. \n\nSource: \nRecombinant corresponding to chicken Leptin, expressed in E.coli.\n\nMolecular Weight:\n~16kD\n\nBiological Activity:\nActive as evidenced by inducing proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its activity is however 5-10 fold lower as compared to mammalian leptins\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

136920

Size

100ug

Form

Supplied as a lyophilized powder in 0.0045mM NaHCO3. Reconstitute with sterile 18Mo-cm H2O to 100ug/ml.

Purity

~95% (RP-HPLC and SDS-PAGE)

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