Leptin, Recombinant, Human (LEP, FLJ94114, Obese Protein, Obesity Factor, OB, OBS)

Source:\nRecombinant corresponding to 147aa of human Leptin expressed in E.coli is a single, non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~16.2kD\n\nBiological Activity:\nThe ED50, calculated by the leptin-dependent stimulation of human OB-R transfected mouse BaF3 indicator cells is 0.5-1.6nug/ml\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.878 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Leptin as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1670-17K

Size

1ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~95% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Human leptin: an adipocyte hormone with weight-regulatory and endocrine functions. Semin Vasc Med 2005 Feb;5(1):15-24. 2. The long-term effect of recombinant methionyl human leptin therapy on hyperandrogenism and menstrual function in female and pituitary function in male and female hypoleptinemic lipodystrophic patients. Metabolism 2005 Feb;54(2):255-63. 3. Association analysis of the Gln223Arg polymorphism in the human leptin receptor gene, and traits related to obesity in Mexican adolescents. J Hum Hypertens 2005 May;19(5):341-6. 4. Effect of administration of recombinant human leptin during the neonatal period on the plasma concentration and gene expression of leptin in the piglet. Biol Neonate 2005;87(1):1-7. 5. Recombinant methionyl human leptin administration activates signal transducer and activator of transcription 3 signaling in peripheral blood mononuclear cells in vivo and regulates soluble tumor necrosis factor-alpha receptor levels in humans with relative leptin deficiency. J Clin Endocrinol Metab 2005 Mar;90(3):1625-31. 6. Recombinant methionyl human leptin administration to achieve high physiologic or pharmacologic leptin levels does not alter circulating infla mmatory marker levels in humans with leptin sufficiency or excess. J Clin Endocrinol Metab 2005 Mar;90(3):1618-24.