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Leptin, Recombinant, Mouse (Ob, Obese Protein, Obesity Factor)

Cat no: L1670-15H

Leptin, Recombinant, Mouse (Ob, Obese Protein, Obesity Factor)

Leptin is a 16kD nonglycosylated hormone that is produced in mature adipocytes. Leptin acts \nprimarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.\n \nMolecular Weight : 16kD (147 amino acids)\n \nSequences:\nMVPIQKVQDD/ TKTLIKTIVT/ RINDISHTQS/ VSAKQRVTGL/ DFIPGLHPIL/ SLSKMDQTLA/ VYQQVLTSLP/ SQNVLQIAND/ LENLRDLLHL/ LAFSKSCSLP/ QTSGLQKPES/ LDGVLEASLY/ STEVVALSRL/ QGSLQDILQQ/ LDVSPEC

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SPECIFICATIONS

Catalog Number

L1670-15H

Size

100ug

Form

Supplied as a liquid in PBS, pH 7.4

Purity

(same/more than)95% by SDS PAGE

References

1. Leclercq,I.A., et al (2003) Gastroenterology 124(5):1451-1464. 2. Anini,Y., et al (2003) Diabetes. 52(2):252-259. 3. Hyogo, H., et al (2002) J. Biol. Chem. 277(37):34117-34124. 4. Zhang, Y., et al (1994) Nature 372(6505):425-432. 5. Yamauchi. T., et al (2002) Nature Medicine 8(11):1288-1295.

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