Leptin, Recombinant, Rat (Ob, Obese Protein, Obesity Factor)

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. n addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses. \n\nBiological Activity: rRLeptin is fully biologically active when compared to standards. The ED50, calculated by the leptin-dependent stimulation of Human OB-R transfected murine BaF3 indicator cells is 0.35-0.06ng/ml.\n\nAA Sequence:\nMVPIHKVQDD TKTLIKTIVT RINDISHTQS VSARQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQILTSLP SQNVLQIAHD LENLRDLLHL LAFSKSCSLP QTRGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDLSPEC \n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, aliquot and store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1670-15L

Size

200ug

Form

Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Purity

~95% Analysis by RP-HPLC; Reducing and non-reducing SDS-PAGE.

References

1. Determination of rat leptin activity in vitro using a novel luciferase reporter assay. Mol Cells 2001 Aug 31;12(1):131-6\n2. Recombinant expression of biologically active rat leptin in Escherichia coli. Protein Expr Purif 2001 Jun;22(1):60-9\n3. Radioimmunoassay of rat leptin: sexual dimorphism reversed from humans. Clin Chem 1998 Mar;44(3):565-70\n4. Molecular cloning of rat leptin receptor isoform complementary DNAs--identification of a missense mutation in Zucker fatty (fa/fa) rats. Biochem Biophys Res Commun 1996 Aug 5;225(1):75-83