Leptin, Triple Antagonist, Recombinant, Mouse (Ob, Obese Protein, Obesity Factor)

Leptin inhibits food intake and stimulates energy expenditure. Leptin also has thermogenic actions and regulates enzymes of fatty acid oxidation. Severe hereditary obesity in rodents and humans is caused by defects in leptin production. In addition to its critical role in the physiologic regulation of body weight leptin has a variety of other physiologic and pathologic functions resembling those of cytokines. These functions include the regulation of hematopoiesis, angiogenesis, wound healing, inflammation, and immune responses.\n\nRecombinant mouse leptin, one polypeptide chain containing 146aa and additional Ala at the N-terminus and having a molecular mass of ~16kD. mLEP was mutated, resulting in the L39A/D40A/F41A mutant.\n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and was found to be Ala-Val-Pro-Ile-Gln\n\nDimers and Aggregates: \nThe purified mLEP triple antagonist (16K) consists of > 99% monomers as determined by gel-filtration chromatography.\n\nBiological Activity: \nMurine LEP triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. It also inhibits various leptin effects in several in vitro bioassays.\n\nEndotoxin: \nLess than 0.1ng/ug (IEU/ug) of mLEP triple antagonist\n\nProtein Content: \nProtein quantization was carried out by UV spectroscopy at 280nm using the absorbency value of 0.21 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).\n\nSolubility: It is recommended to reconstitute the lyophilized mLEP mutant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8-9, not less than 100ug/ml, which can then be further diluted with other aqueous solutions.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L1670-16H

Size

20ug

Form

Supplied as a sterile-filtered, lyophilized powder in 0.003mM NaHCO3.

Purity

Purified by proprietary chromatographic techniques. (same/more than) 98.0% as determined by: (a) Gel filtration analysis. (b) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

References

1. Relationship between soluble leptin receptor, leptin, lipid profiles and anthropometric parameters in overweight and obese Thai subjects. J Med Assoc Thai 2005 Feb;88(2):220-7.\n2. Plasma leptin concentration increases early during highly active antiretroviral therapy for acquired immunodeficiency syndrome, independent of body weight. J Endocrinol Invest 2005 Mar;28(3):RC1-3.\n3. The roles of leptin and adiponectin: a novel paradigm in adipocytokine regulation of liver fibrosis and stellate cell biology. Am J Pathol 2005 Jun;166(6):1655-69.\n4. Leptin therapy does not affect inflammatory markers. J Clin Endocrinol Metab 2005 Jun;90(6):3803; author reply 3803.\n5. Leptin inhibits apolipoprotein M transcription and secretion in human hepatoma cell line, HepG2 cells. Biochim Biophys Acta 2005 May 15;1734(2):198-202.\n6. A retinoid X receptor antagonist, HX531, improves leptin resistance without increasing plasma leptin level in KK-Ay mice under normal dietary conditions. Metabolism 2005 May;54(5):573-8.