LIF, Recombinant, Human (Leukemia Inhibitory Factor)

Based on its helical structure, LIF (Leukemia Inhibitory Factor) is considered a member of the Interleukin-6 family of cytokines. Functionally, it has been implicated in a many physiological processes including development, hematopoiesis, bone metabolism, and inflammation. Some cell types known to express LIF include activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, regenerating skeletal muscle, mast cells, and fibroblasts. \n\nSource:\nRecombinant protein corresponding to aa24-202 from human LIF, expressed in E.coli.\n(Accession# P15018)\n\nMolecular Weight: \n~19.6kD\n\nBiological Activity:\nMeasured by its ability to induce IL-6 secretion by M1 mouse myeloid leukemia cells.\nThe ED50 for this effect is ~0.5ng/ml.\n \nEndotoxin: \n<0.10 EU per 1ug of protein by the LAL method\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

167674

Size

25ug

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS, BSA. Reconstitute with sterile PBS to 100ug/ml.

Purity

~95% (SDS-PAGE)

References

1. Gough, N.M. et al. (1988) Proc. Natl. Acad. Sci. USA 85:2623. 2. Moreau, J.F. et al. (1988) Nature 336:690. 3. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51. 4. Metcalfe, S.M. (2011) Genes Immun. 12:157. 5. Voyle, R.B. et al. (1999) Exp. Cell Res. 249:199. 6. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680. 7. Tomida, M. et al. (1993) FEBS lett. 334:193. 8. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319. 9. Slaets, H. et al. (2010) Trends Mol. Med. 16:493. 10. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145. 11. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977. 12. Duluc, D. et al. (2007) Blood 110:4319.