LIF, Recombinant, Human (Leukemia Inhibitory Factor, hLIF)

Leukemia Inhibitory Factor (LIF) is a 20 kDa pleiotrophic factor belonging to the IL-6 superfamily of cytokines (1). LIF is expressed in a number of tissues and cell types. The LIF receptor is a heterodimer comprised of LIF-R (gp190) and gp130, a common signal transducer for IL-6-type cytokines (1). Depending on cell type and context, LIF/LIF-R can activate Erk, PI3K, and Jak1/Stat1/3 pathways (1,2). LIF has a diverse array of biological activities. Murine embryonic stem cells are dependent on LIF for pluripotency and self-renewal in vitro (1). Exercise- induced LIF secretion in muscle induces myoblast proliferation, suggesting that LIF may play a role in exercise-induced muscle hypertrophy (2). LIF also negatively regulates Th2 and Th17 cell differentiation (3,4).\n\nSource:\nRecombinant protein corresponding to human LIF, expressed in E. coli.\n\nMolecular Weight: \n~20kD\n\nBiological Activity:\nDetermined in a TF-1 cell proliferation assay. The ED50 of each lot is 0.2-1.5ng/ml.\n\nEndotoxin:\n<0.01ng/1ug\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile buffer or ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137462

Size

10ug

Form

Supplied as a lyophilized powder in PBS, pH 7.2, 20ug BSA/ug recombinant LIF. Reconstitute in sterile PBS.

Purity

~98% (SDS-PAGE)

References

Mathieu, M.E. et al. (2012) Stem Cell Rev 8, 1-15. 2. Broholm, C. and Pedersen, B.K. (2010) Exerc Immunol Rev 16, 77-85. 3. Cao, W. et al. (2011) Immunity 35, 273-84. 3. Ullah, U. et al. (2012) Sci Rep 2, 464.