Limax flavus (LFA) (AP)

Pure Limax flavus lectin (LFA) from garden slug, Alkaline Phosphatase conjugated.\n\nLectins are derived from the extracts of plants, animals, viruses and microorganisms and are known to agglutinate red blood cells. These agglutinins can select cell types according to blood group activities utilizing sugar-binding mechanisms. \n\nLectins form precipitates with glycoconjugates and are useful for identifying or separating oligosaccharides with identical sugar compositions such as galactose, mannose or glucose.\n\nCarbohydrate Specificity: Sialic acid (generic term for derivatives of N-acetylneuraminic acid and N-glycolylneuraminic acid).\n\nInhibitory Carbohydrate: N-Acetylneuraminic acid and N-Glycolylneuraminic acid\n\nActivity: 10-15ug/ml will agglutinate type O human erythrocytes.\n\nChemical Used for Conjugation: Alkaline Phosphatase.\n\nStorage and Stability: \nMay be stored at 4 degrees C before opening. DO NOT FREEZE! Stable at 4 degrees C as an undiluted liquid. Dilute only prior to immediate use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.\nFreezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Alkaline phosphatase conjugates are sensitive to light.

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SPECIFICATIONS

Catalog Number

L2217-50C

Size

1mg

Form

Supplied as a liquid in 0.05M Tris, 0.3M sodium chloride, pH 7.5. Dilute with 0.05M Tris, 0.1 sodium chloride, pH 7.5, 20-50% glycerol. Labeled with Alkaline Phosphatase (AP).

Purity

Purified

References

1. Miller, R.L. (1987) Meth. Enzymol. 138:527-536, 2. Ravindranath, M.H. and Paulson, J.C. (1987) Meth. Enzymol. 138:520-527, 3. Roth, J., et al. (1985) Cell. 43:287-295, 4. Biotechs: Sialic Acid and Related Products. Summer 1991. EY Laboratories, Inc.

Alternative Names

Garden Slug