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Lin28, Recombinant Human, His-tag, aa42-209

Cat no: L2280-49

Lin28, Recombinant Human, His-tag, aa42-209

Lin-28 acts as a 'translational enhancer', driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis. It is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem (iPS) cells from human fibroblasts. It has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Recombinant Lin28 protein was expressed in E. coli and purified by using conventional chromatography techniques.\n\nSource:\nRecombinant corresponding to human Lin28, his-tagged, expressed in E. coli. \n\nAA sequence:\nMGSSHHHHHH SSGLVPRGSH RSMGICKWFN VRMGFGFLSM TARAGVALDP PVDVFVHQSK LHMEGFRSLK EGEAVEFTFK KSAKGLESIR VTGPGGVFCI GSERRPKGKS MQKRRSKGDR CYNCGGLDHH AKECKLPPQP KKCHFCQSIS HMVASCPLKA QQGPSAQGKP TYFREEEEEI HSPTLLPEAQ N\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L2280-49

Size

50ug

Reactivities

Hum

Form

Supplied as a liquid in 20mM Tris-HCl, pH8.0, 10% glycerol, 0.1M NaCl.

Purity

~90% (SDS-PAGE) Purified by immunoaffinity chromatography.

References

1.Moss E.G., et al. (2003) Dev. Biol. 258:432-442 2.Heo I., et al. (2009) Cell. 138:696-708.

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