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Lin28B, Recombinant, Human, aa1-250, His-Tag (Protein Lin-28 Homolog B, CSDD2)

Cat no: 138686

Lin28B, Recombinant, Human, aa1-250, His-Tag (Protein Lin-28 Homolog B, CSDD2)

Lin28B belongs to the lin-28 family. LIN28 protein is a microRNA-binding protein that binds to and enhances the translation of the IGF-2 (insulin-like growth factor 2) mRNA. Lin28B is acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. \n\nSource:\nRecombinant protein corresponding to aa1-250 from human Lin28B at N-terminal, fused to His-tag expressed in E.coli. \n\nMolecular Weight: \n~29.5kD (273aa)\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MGS KLFMEGFRSL KEGEPVEFTF KKSSKGLESI RVTGPGGSPC LGSERRPKGK TLQKRKPKGD RCYNCGGLDH HAKECSLPPQ PKKCHYCQSI MAEGGAS KGGGEEPGKL PEPAEEESQV LRGTGHCKWF NVRMGFGFIS MINREGSPLD IPVDVFVHQS\nMHMVANCPHK NVAQPPASSQ GRQEAESQPC TSTLPREVGG GHGCTSPPFP QEARAEISER SGRSPQEASS TKSSIAPEEQ SKKGPSVQKR KKT\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

138686

Size

10ug

Form

Supplied as a liquid in 20mM tris-HCl buffer, pH 8.0, 0.2M sodium chloride, 2mM DTT, 10% glycerol.

Purity

~85% (SDS-PAGE)

References

1. Heo I., et al. (2008) Mol. Cell 32:276-284 2. Richards M, et al. (2004) Stem Cells 22 (1): 51

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