LinKine™ HRP Labeling Kit

Activated Peroxidase is horseradish peroxidase (HRP) enzyme, whose native carbohydrates (sugars) have been gently oxidized with periodate to produce amine-reactive aldehyde groups. These carbonyls of Activated Peroxidase spontaneously and efficiently crosslink with primary amines on antibody or other proteins. The method is more effective than other amine-reactive chemistries, such as glutaraldehyde coupling, which often causes polymerization and a greater degree of conjugate inactivation. The pre-activated enzyme eliminates the difficulties inherent in preparing and validating activated peroxidase from scratch.

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SPECIFICATIONS

Catalog Number

KTL0100

Size

3*20 micro g, 100 micro g, 3*100 micro g, 1 mg

Additional Info

вЂў Activated HRP вЂ“ periodate-treated, aldehyde-activated horseradish peroxidase, ready for Labeling to antibodies and other proteins at sites of primary amines (e.g., lysines).вЂў High activity HRP вЂ“ enzyme activity is 120 to 200 units/mg; activated enzyme is stable for at least 12 months at -20В°C.вЂў Convenient quantities вЂ“ each 1 mg-quantity of activated enzyme is sufficient for reaction with 1 mg of IgG.вЂў Customizable вЂ“ vary the molar ratios, reaction buffer and pH, and other parameters to acheive conjugates with different levels of HRP incorporation and activity.