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Lipase Assay Kit, BioAssay(TM)

Cat no: L2496-08

Lipase Assay Kit, BioAssay(TM)

Lipase catalyzes the hydrolysis of ester bonds on the glycerol backbone of a lipid substrate. In humans, pancreatic lipase is the key enzyme responsible for breaking down fats in the digestive system by converting triglycerides to monoglycerides and free fatty acids. Human pancreatic lipase and its related protein 2 are the main lipases secreted by the pancreas. In acute pancreatitis, lipase levels can rise 5 to 10-fold within 24 to 48 hours. Increased activities have also been associated with pancreatic duct obstruction, pancreatic cancer, kidney disease, salivary gland inflammation, bowel obstruction, and other pancreatic diseases. Decreased levels may indicate permanent damage to lipase-producing cells in the pancreas.\n\nSimple, direct and automation-ready procedures for measuring lipase activity are very desirable. Lipase Assay is based on an improved dimercaptopropanol tributyrate (BALB) method, in which SH groups formed from lipase cleavage of BALB react with 5,5

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SPECIFICATIONS

Catalog Number

L2496-08

Size

1Kit

References

1. Furukawa, I. et al (1982). Assays of serum lipase by the \"BALB- DTNB method\" mechanized for use with discrete and continuous- flow analyzers. Clin Chem. 28: 110-113.\n2. Lombard, S. et al (2001). Lipolytic activity of ricin from Ricinus sanguineus and Ricinus communis on neutral lipids. Biochem J. 358: 773

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