Lipase

Pancreatic lipase (PNLIP) fulfills a key function in dietary fat absorption by hydrolyzing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids. By screening a human pancreas cDNA library with degenerate oligonucleotides designed from the amino acid sequences of human, canine, and porcine PNLIP, Giller et al. (1992) isolated cDNAs encoding 2 novel PNLIP-related proteins, PNLIPRP1 (604422) and PNLIPRP2, which they called PLRP1 and PLRP2, respectively. The predicted 469-amino acid PNLIPRP2 protein contains a 17-amino acid signal peptide and the conserved residues of the lipase catalytic triad and oxy-anion hole. Among known lipases, PNLIPRP2 was most related to a mouse T-cell lipase, with 74% amino acid identity. PNLIPRP2 has 65% amino acid sequence identity with PNLIP, 63% identity with PNLIPRP1, and 20 to 30% identity with human gastric lipase, lipoprotein lipase, and hepatic lipase.\n\nApplications: \nSuitable for use in ELISA or antigenic applications in immunological protocols. Other applications not tested.\n\nRecommended Dilutions:\nOptimal dilutions to be determined by the researcher.\n\nAffinity Constant:\n~1.1x10e9L/mole\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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