Lipid Peroxidation, Colorimetric Assay Kit, BioAssay(TM)

Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals, and is used as an indicator of oxidative stress in cells and tissues. Lipid peroxides are unstable and decompose to form a complex series of compounds including reactive carbonyl compounds. Polyunsaturated fatty acid peroxides generate malondialdehyde (MDA) and 4-hydroxyalkenals (HAE) upon decomposition.\n\nMeasurement of malondialdehyde and 4-hydroxyalkenals has been used as an indicator of lipid peroxidation. This method is designed to assay either MDA alone (in hydrochloric acid) or MDA in combination with 4-hydroxyalkenals (in methanesulfonic acid.)\n\nPrinciple of the Assay:\nThis assay is based on the reaction of a chromogenic reagent, N-methyl-2-phenylindole (R1), with MDA and 4-hydroxyalkenals at 45 degrees C. One molecule of either MDA or 4-hydroxyalkenal reacts with 2 molecules of reagent R1 to yield a stable chromophore with maximal absorbance at 586nm.\n\nKit Components:\nL2496-30A: Reagent R1, 3 x18ml. Supplied as a liquid , N-methyl-2-phenylindole in acetonitrile\nL2496-30B: Reagent R2, 1x16.5ml. Supplied as a liquid, Methanesulfonic acid (MSA)\nL2496-30C: MDA Standard (S2), 1x1ml. Supplied as a liquid, 1,1,3,3-Tetramethoxypropane in Tris-HCl\nL2496-30D: Diluent, 1x30ml. Supplied as a liquid Ferric Iron in Methanol\n\nStorage and Stability:\nStore all components at 4 degrees C. Stable for at least 6 months

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SPECIFICATIONS

Catalog Number

L2496-30

Size

1Kit

References

1. Esterbauer, H., Schaur, R.J. and Zollner, H. (1991) Chemistry and Biochemistry of 4-Hyroxynonenal, Malonaldehyde and Related Aldehydes; Free Rad. Biol. Med. 11:81-128.2. Botsoglou, N.A. et al (1994) Rapid, Sensitive, and Specific Thiobarbituric Acid Method for Measuring Lipid Peroxidation in Animal Tissue, Food and Feedstuff Samples; J. Agric. Food Chem. 42:1931-1937.3. Carbonneau, M.A. et al (1991) Free and Bound Malondialdehyde Measured as Thiobarbituric Acid Adduct by HPLC in Serum and Plasma; Clin. Chem. 37:1423-1429.4. Bull, A.W. and Marnett, L.J. (1985) Determination of Malondialdehyde by Ion-Pairing High-Performance Liquid Chromatography; Analyt. Biochem. 149:284-290.5. Liu, J. et al (1997) Assay of Aldehydes from Lipid Peroxidation: Gas Chromatography-Mass Spectrometry Compared to Thiobarbituric Acid; Analyt. Biochem. 245: 161-166.