MALDI-IMS is a powerful tool for examining the distribution of lipids in tissues. As an ion source, MALDI is particularly well-suited for thin tissue sections and is extensively employed for analysis of tissue samples. The benefits of MALDI-IMS include: 1) Simplified extraction steps; 2) Reduced sample loss; 3) Omission of complex deduction processes; 4) Provision of detailed spatial organization and distribution data.