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Lipoamide Dehydrogenase, aa36-509, Recombinant, Human (Diaphorase, Dihydrolipoamide Dehydrogenase, Dihydrolipoyl Dehydrogenase Mitochondrial, DLD, DLDH, E3, Glycine Cleavage System Protein L, GCSL, Lipoamide Reductase, Lipoyl Dehydrogenase, LAD, PHE3)

Cat no: L2498-15H

Lipoamide Dehydrogenase, aa36-509, Recombinant, Human (Diaphorase, Dihydrolipoamide Dehydrogenase, Dihydrolipoyl Dehydrogenase Mitochondrial, DLD, DLDH, E3, Glycine Cleavage System Protein L, GCSL, Lipoamide Reductase, Lipoyl Dehydrogenase, LAD, PHE3)

DLD (Dihydrolipoamide dehydrogenase), also known as GCSL (glycine cleavage system L protein), is a component of the glycine cleavage system as well as of the alpha ketoacid dehydrogenase complexes. DLD is a flavin-dependent oxidoreductase and functions as a component of the a-keto acid dehydrogenase, the pyruvate dehydrogenase, the a-ketoglutarate dehydrogenase, the branched-chain a-keto acid dehydrogenase and as the L protein in the mitochondrial glycine cleavage system. Mutations in DLD protein can result in MSUD (maple syrup urine disease) and congenital infantile lactic acidosis. Recombinant human DLD protein, fused to His-tag at N-terminus, was expressed in E. coli.\n\nSource: \nRecombinant corresponding to aa36-509 of human DLD 6x His tagged expressed in E. coli.\n\nAA Sequence:\nMRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMADQ PIDADVTVIG SGPGGYVAAI KAAQLGFKTV CIEKNETLGG TCLNVGCIPS KALLNNSHYY HMAHGKDFAS RGIEMSEVRL NLDKMMEQKS TAVKALTGGI AHLFKQNKVV HVNGYGKITG KNQVTATKAD GGTQVIDTKN \nILIATGSEVT PFPGITIDED TIVSSTGALS LKKVPEKMVV IGAGVIGVEL GSVWQRLGAD VTAVEFLGHV GGVGIDMEIS KNFQRILQKQ GFKFKLNTKV TGATKKSDGK IDVSIEAASG GKAEVITCDV LLVCIGRRPF TKNLGLEELG IELDPRGRIP VNTRFQTKIP NIYAIGDVVA GPMLAHKAED EGIICVEGMA GGAVHIDYNC VPSVIYTHPE VAWVGKSEEQ LKEEGIEYKV GKFPFAANSR AKTNADTDGM VKILGQKSTD \nRVLGAHILGP GAGEMVNEAA LALEYGASCE DIARVCHAHP TLSEAFREAN LAASFGKSIN F\n\nApplications: \nSuitable for use in Western Blot. Other applications not tested.\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

L2498-15H

Size

100ug

Applications

WB

Form

Supplied as a liquid in 20mM Tris HCl, pH 8.0, 1mM DTT, 0.1M NaCl, 10% glycerol.

Purity

~ 95% (SDS-PAGE). Purified by using conventional chromatography techniques.

References

1. Giannopoulou E., et al. (2009) Anticancer Res. 29(12):5077-82. 2. Daee DL., et al. (2009) Proc Natl Acad Sci U S A. 107(1):157-62.

Alternative Names

EC=1.8.1.4, E3 Component of Pyruvate Dehydrogenase Complex 2 Oxo Glutarate Complex Branched Chain Keto Acid Dehydrogenase Complex

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