Lipocalin 2, Recombinant, Human (HNL, NGAL, Neutrophil Gelatinase Associated Lipocalin, Oncogene 24p3, Neutrophil gelatinase-associated lipocalin precursor P25, alpha-2 microglobulin-related subunit of MMP-9)

Recombinant Human Lipocalin 2 also called Neutrophil Gelatinase Associated Lipocalin (NGAL) belongs to a family of lipocans which include 25 proteins (including alpha1-microglobulin and beta-lactoglobulin), which are characterized by their ability to bind small lipophilic substances in their hydrophobic core. They thereby serve as transporters of substances like retinal, biliverdins & prostaglandins. There are indications that NGAL is involved in modulation of the inflammatory response and is found in the plasma of patients after stroke. Human neutrophil gelatinase-associated lipocalin (NGAL), and its mouse analog 24p3, are members of the lipocalin family of small secreted proteins. These proteins are up-regulated in a number of pathological conditions, including cancers, and may function as transporters of essential factors.\n\nRecombinant Human NGAL is expressed in E. coli having a molecular weight of 28.1kD fused to an amino terminal hexahistidine tag.\n\nBiological Activity:\nNot tested. \n\nApplications: \nSuitable for use in Western Blot, ELISA and Immunoprecipitation. Other applications have not been tested.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, aliquot and store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

L2499

Size

2ug

Applications

ELISA, IP, WB

Form

Supplied as a liquid in PBS, 50% glycerol.

Purity

(same/more than) 95% (SDS-PAGE). Single band on Western Blot.

References

1. Identification of neutrophil gelatinase-associated lipocalin (NGAL) as a discriminatory marker of the hepatocyte-secreted protein1. Kjeldsen L et al (1993) Isolation and primary structure of NGAL, a novel protein associated with human neutrophil gelatinase. J Biol Chem; 268:10425-32. 2. Kjeldsen L et al (1996) Characterization of two ELISAs for NGAL, a newly described lipocalin in human neutrophils. J Immunol Meth 198:155-64. 3. Mishra J, Dent C, Tarabishi R, Mitsnefes MM, Ma Q, Kelly C, Ruff SM, Zahedi K, Shao M, Bean J, Mori K, Barasch J, Devarajan P (2005) Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 365(9466):1231-1238. response to IL-1beta: a proteomic analysis. Biotechnol Bioeng 2005 Aug 20;91(4):502-15. 4. Neutrophil gelatinase-associated lipocalin (NGAL) as a biomarker for acute renal injury after cardiac surgery. Lancet 2005 Apr 2-8;365(9466):1231-8 5. [Cloning and identification of 5'-untranslated region (UTR) and 3'-untranslated region of neutrophil gelatinase-associated lipocalin (NGAL) gene from esophageal carcinoma cell line SHEEC] Ai Zheng 2003 Feb;22(2):143-7. 6. The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition. Mol Cell 2002 Nov;10(5):1033-43.