Lipoprotein, High Density (HDL) and Lipoprotein, Low Density (LDL)/Lipoprotein, Very Low Density (VLDL) BioAssay(TM) Kit, Colorimetric/Fluorimetric

Cholesterol concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors.\n\nSimple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lem/ex=585/530nm is directly proportional to total cholesterol concentration in the sample.\n\nApplications:\nDirect Assays: HDL and LDL/VLDL cholesterol in serum samples.\nPharmacology: evaluation of drugs on cholesterol metabolism.\n\nKey Features:\nSensitive and accurate. Linear detection range in 96-well plate: 1 to 100mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.\nConvenient. Room temperature assay. No 37 degrees C heater is needed.\n\nKit Contents: (100 assays in 96-well plates)\nPBS: 2

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SPECIFICATIONS

Catalog Number

L2600-57A

Size

1Kit

References

1. Tam, J, et al. (2010). Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity. J Clin Invest. 120 (8): 2953-66. 2. Nagajyothi, F. et al. (2011). Trypanosoma cruzi utilizes the host low density lipoprotein receptor in invasion. PLoS Negl Trop Dis. 1; 5(2): e953.\n3. Parveen, T.D. et al. (2011). Antihyperlipidemic Activity of the Methanolic extract from the Stems of Tinospora cordifolia on Sprague dawley rats. Der Pharmacia Sinica, 2011, 2 (1): 104-109.